cdna aliquots (Bio-Rad)
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cdna aliquots
Cdna Aliquots, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+aliquots/Sample+Templates/pm22545806__cb3000474_si_001-35-1-22
Average 90 stars, based on 20 article reviews
Cdna Aliquots, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+aliquots/Sample+Templates/pm22545806__cb3000474_si_001-35-1-22
Average 90 stars, based on 20 article reviews
cdna aliquots - by Bioz Stars,
2026-09
90/100 stars
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Purification:Article Title: Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage. Article Snippet: RT Quantitative Real-Time PCR. hMSCs were lyzed in TRIZOL reagent (Invitrogen) to isolate the total RNA, and a TurboDNA free kit (Ambion, Illkirch-Graffenstaden, France) was used to remove contaminating DNA from the RNA preparations. .. Two micrograms of purified total RNA was used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific, Illkirch-Graffenstaden, France) and random primers (Thermo Scientific). Article Title: Directing hMSCs fate through geometrical cues and mimetics peptides. Article Snippet: TurboDNA free kit (Ambion) was used to remove contaminating DNA from RNA preparations. .. 2 μg of purified total RNA were used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific) and random primers (Thermo Scientific). Reverse Transcription:Article Title: Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage. Article Snippet: RT Quantitative Real-Time PCR. hMSCs were lyzed in TRIZOL reagent (Invitrogen) to isolate the total RNA, and a TurboDNA free kit (Ambion, Illkirch-Graffenstaden, France) was used to remove contaminating DNA from the RNA preparations. .. Two micrograms of purified total RNA was used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific, Illkirch-Graffenstaden, France) and random primers (Thermo Scientific). Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice. Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Directing hMSCs fate through geometrical cues and mimetics peptides. Article Snippet: TurboDNA free kit (Ambion) was used to remove contaminating DNA from RNA preparations. .. 2 μg of purified total RNA were used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific) and random primers (Thermo Scientific). Amplification:Article Title: Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage. Article Snippet: RT Quantitative Real-Time PCR. hMSCs were lyzed in TRIZOL reagent (Invitrogen) to isolate the total RNA, and a TurboDNA free kit (Ambion, Illkirch-Graffenstaden, France) was used to remove contaminating DNA from the RNA preparations. .. Two micrograms of purified total RNA was used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific, Illkirch-Graffenstaden, France) and random primers (Thermo Scientific). Article Title: A fragment of the hypophosphatemic factor, MEPE, requires inducible cyclooxygenase-2 to exert potent anabolic effects on normal human marrow osteoblast precursors. Article Snippet: MEPE, 56.6 kDaprotein isolated from tumors associatedwith hypophosphatemic osteomalacia, increases renal phosphate excretion and is expressed in normal human bone cells.. AC-100, a central 23-amino acid fragment of MEPE, containsmotifs that are important in regulating cellular activities in the bonemicroenvironment.. Thus, we assessed in vitro effects of AC-100 on multipotential normal human marrow stromal (hMS) cells that have the capacity to differentiate into mature osteoblasts. Article Title: Directing hMSCs fate through geometrical cues and mimetics peptides. Article Snippet: TurboDNA free kit (Ambion) was used to remove contaminating DNA from RNA preparations. .. 2 μg of purified total RNA were used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific) and random primers (Thermo Scientific). SYBR Green Assay:Article Title: Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage. Article Snippet: RT Quantitative Real-Time PCR. hMSCs were lyzed in TRIZOL reagent (Invitrogen) to isolate the total RNA, and a TurboDNA free kit (Ambion, Illkirch-Graffenstaden, France) was used to remove contaminating DNA from the RNA preparations. .. Two micrograms of purified total RNA was used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific, Illkirch-Graffenstaden, France) and random primers (Thermo Scientific). Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice. Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: A fragment of the hypophosphatemic factor, MEPE, requires inducible cyclooxygenase-2 to exert potent anabolic effects on normal human marrow osteoblast precursors. Article Snippet: MEPE, 56.6 kDaprotein isolated from tumors associatedwith hypophosphatemic osteomalacia, increases renal phosphate excretion and is expressed in normal human bone cells.. AC-100, a central 23-amino acid fragment of MEPE, containsmotifs that are important in regulating cellular activities in the bonemicroenvironment.. Thus, we assessed in vitro effects of AC-100 on multipotential normal human marrow stromal (hMS) cells that have the capacity to differentiate into mature osteoblasts. Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Directing hMSCs fate through geometrical cues and mimetics peptides. Article Snippet: TurboDNA free kit (Ambion) was used to remove contaminating DNA from RNA preparations. .. 2 μg of purified total RNA were used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific) and random primers (Thermo Scientific). Article Title: Article Snippet: RNA (1 μg) was reverse transcribed using random primers (#48190-011, Life Technologies) and SuperScriptIII reverse transcriptase (#18080-044, Life Technologies). mRNA levels of fkbp5 and pck1 were determined by real-time qPCR (StepOne Plus, Applied Biosystems), following the manufacturer’s instructions. .. First-strand Real-time Polymerase Chain Reaction:Article Title: Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage. Article Snippet: RT Quantitative Real-Time PCR. hMSCs were lyzed in TRIZOL reagent (Invitrogen) to isolate the total RNA, and a TurboDNA free kit (Ambion, Illkirch-Graffenstaden, France) was used to remove contaminating DNA from the RNA preparations. .. Two micrograms of purified total RNA was used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific, Illkirch-Graffenstaden, France) and random primers (Thermo Scientific). Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice. Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice Article Snippet: .. Total RNA was then converted into cDNA via reverse transcription using iScriptTM Reverse Transcription Supermix (Bio-Rad, Canada) and a T100 Thermal Cycler (Bio-Rad, Canada) and Article Title: Directing hMSCs fate through geometrical cues and mimetics peptides. Article Snippet: TurboDNA free kit (Ambion) was used to remove contaminating DNA from RNA preparations. .. 2 μg of purified total RNA were used to synthesize cDNA using Thermo Scientific Maxima Reverse Transcriptase (Thermo Scientific) and random primers (Thermo Scientific). Polymerase Chain Reaction:Article Title: A fragment of the hypophosphatemic factor, MEPE, requires inducible cyclooxygenase-2 to exert potent anabolic effects on normal human marrow osteoblast precursors. Article Snippet: MEPE, 56.6 kDaprotein isolated from tumors associatedwith hypophosphatemic osteomalacia, increases renal phosphate excretion and is expressed in normal human bone cells.. AC-100, a central 23-amino acid fragment of MEPE, containsmotifs that are important in regulating cellular activities in the bonemicroenvironment.. Thus, we assessed in vitro effects of AC-100 on multipotential normal human marrow stromal (hMS) cells that have the capacity to differentiate into mature osteoblasts. Article Title: Article Snippet: RNA (1 μg) was reverse transcribed using random primers (#48190-011, Life Technologies) and SuperScriptIII reverse transcriptase (#18080-044, Life Technologies). mRNA levels of fkbp5 and pck1 were determined by real-time qPCR (StepOne Plus, Applied Biosystems), following the manufacturer’s instructions. .. First-strand |